PCR System – Biotechnics International

Qiagene Rotor Gene PCR System

The Rotor-Gene Q — designed to drive designed to drive your research forward

QIAGEN’s real-time PCR cycler, the Rotor-Gene Q, combines multiple optimized design features to provide the outstanding performance and reliable results that your research demands. Together with optimized QIAGEN kits for real-time PCR, the Rotor-Gene Q enables streamlined analysis for a wide range of applications.

Benefits of the Rotor-Gene Q

Outstanding thermal and optical performance due to rotary format
An unmatched optical range spanning UV to infrared wavelengths
State-of-the art analyses supported by user-friendly software
Low maintenance and maximum convenience due to robust design
High performance in multiple applications with QIAGEN kits and assays

Comprehensive Application Range

The Rotor-Gene Q combined with optimized QIAGEN kits addresses all real-time PCR and high-resolution melting (HRM) applications, including:

The Rotor-Gene Q-designed to Drive your research forward

Quantitative, real-time PCR is a precision science that places high demands on the instrument, chemistry, and software. High thermal and optical uniformity, short equilibration times, and fast ramping rates are critical for precise and rapid quantitative analysis, Sensitivity, speed, and specificity are also highly dependent on the performance of the DNA polymerase and reaction components. QIAGEN’s real-time PCR cycler, the Rotor-Gene Q, combines multiple optimized design features to provide the outstanding performance and reliable results that your research demands. Together with optimized QIAGEN kits for real-time PCR, the Rotor-Gene Q enables streamlined analysis for a wide range of applications.

Unrivaled Optical Range Enables Multiple Applications

Whether your assay is based on intercalating dyes such as SYBR® Green, probes such as hydrolysis (TaqMan) or hybridization (FRET) probes, or multiplex chemistries, the Rotor-Gene Q meets your requirements. With up to 6 channels spanning UV to infrared wavelengths, the cycler delivers the widest optical range currently available (Table 1). In addition, the software allows you to create new excitation/detection wavelength combinations, which means that the Rotor-Gene Q is compatible with dyes you may use in the future

Table 1. Channels for optical detection

Channel	Excitation (nm)	Detection (nm)	Examples of fluorophores detected
Blue	365+20	460+20	Marina Blue, Edans, Bothell Blue, Alexa Fluor 3.50
Green	4/0-10	510-5	FAM", SYBR Green I, Fluorescein, EverGreen", Alexa Fluor 488
Yellow	530+5	557+5	JOE, VIC, HEX, TET, CAL Fun Gold 540, Yakima Yellow
Orange	585+5	610-5	ROX", CAL Fluor Red 610, Cy 3.5, Texas Red, Alexa Fluor 568
Red	625+5	660-10	Cy5, Qusar 670, LigiCycler Red640, Alex Flunr 633
Crimson	680+5	712 high pass	Quasar 705, LightCycler Red705, Alexa Fluor 680
HRM	460+20	510-5	SYER Green I, SYTO 9, LC Green, LC Green Plus+, EvcGreen

Flexible formats match your workflows

The Rotor-Gene Q supports multiple PCR tube formats to suit a range of needs. Changing the format, by simply switching the snap-fit metal rotor that holds the tubes, takes just seconds. As well as tubes, Rotor-DiscsTM are available, which offer accelerated setup and higher throughput. Rotor-Discs are circular plates of vertically. oriented reaction wells. The Rotor-Disc 100 is the equivalent of a 96-well plate with an additional 4 reference wells. These extra wells can be conveniently used for more reactions or additional controls. Rotor-Discs can be quickly and easily sealed with plastic film using a Rotor-Disc Heat Sealer.

All formats are fully compatible with the QlAgilityTM” instrument for automated reaction setup, Reaction setup for the data shown in this brochure was performed using the QlAgility. Visit www.giagen.com/goto/QlAgility to find out how the QIAgility can fully automate your PCR setup and free up your time.